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sheep polyclonal antibody against nephrin  (R&D Systems)


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    R&D Systems sheep polyclonal antibody against nephrin
    Fig. 4 Hepatocyte-specific overexpression of Klotho ameliorates renal injury and proteinuria in a mouse model of NTS-induced glomerulonephritis. a Left panel: Urinary albumin/creatinine ratio (U-ACR) in WT, Alb-hKL, and Pod-hKL, 0, 1, 3, and 7 days after injection of NTS. $$$$p < 0.0001 vs. Pod-hKL and @@@@p < 0.0001 vs. WT. Right panel: Blood urea nitrogen in the different groups without injury (control mice) or 7 days after injection of NTS (NTS mice). ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT-NTS. b Representative images of PAS (left panels) and TEM (right panels) of kidney cortex from WT-control and WT, Alb-hKL, Pod-hKL 7 days after NTS injection. c Percentage of affected and crescentic glomeruli were quantified from 30 randomly selected glomeruli in the corresponding PAS staining. Tubular casts were assessed over the whole cross-section giving a score between 0, no cast to 5, >80% of the tubules are affected (n = 11 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 11 Pod-hKL-NTS). Quantification of mean glomerular basement membrane (GBM) thickness and the number of slits per μm GBM (n = 5 WT-control; n = 10 WT-NTS; n = 6 Alb-hKL-NTS; n = 8 Pod-hKL-NTS). ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05, @@p < 0.01 vs. WT-NTS and *p < 0.05, ***p < 0.001 vs. Alb-hKL-NTS. d Representative images of immunofluorescent stainings of <t>Nephrin,</t> WT-1, and α-SMA in the different groups. e Quantification of the number of WT-1 positive podocytes in glomeruli (left panel), glomerular area (middle panel), and WT-1 positive podocytes/glomerular area (right panel) (n = 7 WT-control; n = 9 WT-NTS; n = 5 Alb-hKL- NTS; n = 9 Pod-hKL-NTS). α-SMA positive area was counted per cortical cross section (10 sections per sample; n = 6 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 9 Pod-hKL-NTS). ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT vs. WT-NTS and *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. Alb-hKL-NTS. Scale bars: 50 μm, 30 μm, 5 μm (b), 10 μm, 50 μm (d). Statistical significance between groups was determined using one- way or two-way repeated measures ANOVA followed by multiple comparisons.
    Sheep Polyclonal Antibody Against Nephrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep polyclonal antibody against nephrin/product/R&D Systems
    Average 95 stars, based on 129 article reviews
    sheep polyclonal antibody against nephrin - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Soluble Klotho protects against glomerular injury through regulation of ER stress response."

    Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response.

    Journal: Communications biology

    doi: 10.1038/s42003-023-04563-1

    Fig. 4 Hepatocyte-specific overexpression of Klotho ameliorates renal injury and proteinuria in a mouse model of NTS-induced glomerulonephritis. a Left panel: Urinary albumin/creatinine ratio (U-ACR) in WT, Alb-hKL, and Pod-hKL, 0, 1, 3, and 7 days after injection of NTS. $$$$p < 0.0001 vs. Pod-hKL and @@@@p < 0.0001 vs. WT. Right panel: Blood urea nitrogen in the different groups without injury (control mice) or 7 days after injection of NTS (NTS mice). ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT-NTS. b Representative images of PAS (left panels) and TEM (right panels) of kidney cortex from WT-control and WT, Alb-hKL, Pod-hKL 7 days after NTS injection. c Percentage of affected and crescentic glomeruli were quantified from 30 randomly selected glomeruli in the corresponding PAS staining. Tubular casts were assessed over the whole cross-section giving a score between 0, no cast to 5, >80% of the tubules are affected (n = 11 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 11 Pod-hKL-NTS). Quantification of mean glomerular basement membrane (GBM) thickness and the number of slits per μm GBM (n = 5 WT-control; n = 10 WT-NTS; n = 6 Alb-hKL-NTS; n = 8 Pod-hKL-NTS). ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05, @@p < 0.01 vs. WT-NTS and *p < 0.05, ***p < 0.001 vs. Alb-hKL-NTS. d Representative images of immunofluorescent stainings of Nephrin, WT-1, and α-SMA in the different groups. e Quantification of the number of WT-1 positive podocytes in glomeruli (left panel), glomerular area (middle panel), and WT-1 positive podocytes/glomerular area (right panel) (n = 7 WT-control; n = 9 WT-NTS; n = 5 Alb-hKL- NTS; n = 9 Pod-hKL-NTS). α-SMA positive area was counted per cortical cross section (10 sections per sample; n = 6 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 9 Pod-hKL-NTS). ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT vs. WT-NTS and *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. Alb-hKL-NTS. Scale bars: 50 μm, 30 μm, 5 μm (b), 10 μm, 50 μm (d). Statistical significance between groups was determined using one- way or two-way repeated measures ANOVA followed by multiple comparisons.
    Figure Legend Snippet: Fig. 4 Hepatocyte-specific overexpression of Klotho ameliorates renal injury and proteinuria in a mouse model of NTS-induced glomerulonephritis. a Left panel: Urinary albumin/creatinine ratio (U-ACR) in WT, Alb-hKL, and Pod-hKL, 0, 1, 3, and 7 days after injection of NTS. $$$$p < 0.0001 vs. Pod-hKL and @@@@p < 0.0001 vs. WT. Right panel: Blood urea nitrogen in the different groups without injury (control mice) or 7 days after injection of NTS (NTS mice). ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT-NTS. b Representative images of PAS (left panels) and TEM (right panels) of kidney cortex from WT-control and WT, Alb-hKL, Pod-hKL 7 days after NTS injection. c Percentage of affected and crescentic glomeruli were quantified from 30 randomly selected glomeruli in the corresponding PAS staining. Tubular casts were assessed over the whole cross-section giving a score between 0, no cast to 5, >80% of the tubules are affected (n = 11 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 11 Pod-hKL-NTS). Quantification of mean glomerular basement membrane (GBM) thickness and the number of slits per μm GBM (n = 5 WT-control; n = 10 WT-NTS; n = 6 Alb-hKL-NTS; n = 8 Pod-hKL-NTS). ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05, @@p < 0.01 vs. WT-NTS and *p < 0.05, ***p < 0.001 vs. Alb-hKL-NTS. d Representative images of immunofluorescent stainings of Nephrin, WT-1, and α-SMA in the different groups. e Quantification of the number of WT-1 positive podocytes in glomeruli (left panel), glomerular area (middle panel), and WT-1 positive podocytes/glomerular area (right panel) (n = 7 WT-control; n = 9 WT-NTS; n = 5 Alb-hKL- NTS; n = 9 Pod-hKL-NTS). α-SMA positive area was counted per cortical cross section (10 sections per sample; n = 6 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 9 Pod-hKL-NTS). ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT vs. WT-NTS and *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. Alb-hKL-NTS. Scale bars: 50 μm, 30 μm, 5 μm (b), 10 μm, 50 μm (d). Statistical significance between groups was determined using one- way or two-way repeated measures ANOVA followed by multiple comparisons.

    Techniques Used: Over Expression, Injection, Control, Staining, Membrane



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    sheep polyclonal antibody against nephrin  (R&D Systems)
    95
    R&D Systems sheep polyclonal antibody against nephrin
    Fig. 4 Hepatocyte-specific overexpression of Klotho ameliorates renal injury and proteinuria in a mouse model of NTS-induced glomerulonephritis. a Left panel: Urinary albumin/creatinine ratio (U-ACR) in WT, Alb-hKL, and Pod-hKL, 0, 1, 3, and 7 days after injection of NTS. $$$$p < 0.0001 vs. Pod-hKL and @@@@p < 0.0001 vs. WT. Right panel: Blood urea nitrogen in the different groups without injury (control mice) or 7 days after injection of NTS (NTS mice). ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT-NTS. b Representative images of PAS (left panels) and TEM (right panels) of kidney cortex from WT-control and WT, Alb-hKL, Pod-hKL 7 days after NTS injection. c Percentage of affected and crescentic glomeruli were quantified from 30 randomly selected glomeruli in the corresponding PAS staining. Tubular casts were assessed over the whole cross-section giving a score between 0, no cast to 5, >80% of the tubules are affected (n = 11 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 11 Pod-hKL-NTS). Quantification of mean glomerular basement membrane (GBM) thickness and the number of slits per μm GBM (n = 5 WT-control; n = 10 WT-NTS; n = 6 Alb-hKL-NTS; n = 8 Pod-hKL-NTS). ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05, @@p < 0.01 vs. WT-NTS and *p < 0.05, ***p < 0.001 vs. Alb-hKL-NTS. d Representative images of immunofluorescent stainings of <t>Nephrin,</t> WT-1, and α-SMA in the different groups. e Quantification of the number of WT-1 positive podocytes in glomeruli (left panel), glomerular area (middle panel), and WT-1 positive podocytes/glomerular area (right panel) (n = 7 WT-control; n = 9 WT-NTS; n = 5 Alb-hKL- NTS; n = 9 Pod-hKL-NTS). α-SMA positive area was counted per cortical cross section (10 sections per sample; n = 6 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 9 Pod-hKL-NTS). ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT vs. WT-NTS and *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. Alb-hKL-NTS. Scale bars: 50 μm, 30 μm, 5 μm (b), 10 μm, 50 μm (d). Statistical significance between groups was determined using one- way or two-way repeated measures ANOVA followed by multiple comparisons.
    Sheep Polyclonal Antibody Against Nephrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep polyclonal antibody against nephrin/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    sheep polyclonal antibody against nephrin - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier

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    Fig. 4 Hepatocyte-specific overexpression of Klotho ameliorates renal injury and proteinuria in a mouse model of NTS-induced glomerulonephritis. a Left panel: Urinary albumin/creatinine ratio (U-ACR) in WT, Alb-hKL, and Pod-hKL, 0, 1, 3, and 7 days after injection of NTS. $$$$p < 0.0001 vs. Pod-hKL and @@@@p < 0.0001 vs. WT. Right panel: Blood urea nitrogen in the different groups without injury (control mice) or 7 days after injection of NTS (NTS mice). ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT-NTS. b Representative images of PAS (left panels) and TEM (right panels) of kidney cortex from WT-control and WT, Alb-hKL, Pod-hKL 7 days after NTS injection. c Percentage of affected and crescentic glomeruli were quantified from 30 randomly selected glomeruli in the corresponding PAS staining. Tubular casts were assessed over the whole cross-section giving a score between 0, no cast to 5, >80% of the tubules are affected (n = 11 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 11 Pod-hKL-NTS). Quantification of mean glomerular basement membrane (GBM) thickness and the number of slits per μm GBM (n = 5 WT-control; n = 10 WT-NTS; n = 6 Alb-hKL-NTS; n = 8 Pod-hKL-NTS). ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05, @@p < 0.01 vs. WT-NTS and *p < 0.05, ***p < 0.001 vs. Alb-hKL-NTS. d Representative images of immunofluorescent stainings of Nephrin, WT-1, and α-SMA in the different groups. e Quantification of the number of WT-1 positive podocytes in glomeruli (left panel), glomerular area (middle panel), and WT-1 positive podocytes/glomerular area (right panel) (n = 7 WT-control; n = 9 WT-NTS; n = 5 Alb-hKL- NTS; n = 9 Pod-hKL-NTS). α-SMA positive area was counted per cortical cross section (10 sections per sample; n = 6 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 9 Pod-hKL-NTS). ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT vs. WT-NTS and *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. Alb-hKL-NTS. Scale bars: 50 μm, 30 μm, 5 μm (b), 10 μm, 50 μm (d). Statistical significance between groups was determined using one- way or two-way repeated measures ANOVA followed by multiple comparisons.

    Journal: Communications biology

    Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response.

    doi: 10.1038/s42003-023-04563-1

    Figure Lengend Snippet: Fig. 4 Hepatocyte-specific overexpression of Klotho ameliorates renal injury and proteinuria in a mouse model of NTS-induced glomerulonephritis. a Left panel: Urinary albumin/creatinine ratio (U-ACR) in WT, Alb-hKL, and Pod-hKL, 0, 1, 3, and 7 days after injection of NTS. $$$$p < 0.0001 vs. Pod-hKL and @@@@p < 0.0001 vs. WT. Right panel: Blood urea nitrogen in the different groups without injury (control mice) or 7 days after injection of NTS (NTS mice). ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT-NTS. b Representative images of PAS (left panels) and TEM (right panels) of kidney cortex from WT-control and WT, Alb-hKL, Pod-hKL 7 days after NTS injection. c Percentage of affected and crescentic glomeruli were quantified from 30 randomly selected glomeruli in the corresponding PAS staining. Tubular casts were assessed over the whole cross-section giving a score between 0, no cast to 5, >80% of the tubules are affected (n = 11 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 11 Pod-hKL-NTS). Quantification of mean glomerular basement membrane (GBM) thickness and the number of slits per μm GBM (n = 5 WT-control; n = 10 WT-NTS; n = 6 Alb-hKL-NTS; n = 8 Pod-hKL-NTS). ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05, @@p < 0.01 vs. WT-NTS and *p < 0.05, ***p < 0.001 vs. Alb-hKL-NTS. d Representative images of immunofluorescent stainings of Nephrin, WT-1, and α-SMA in the different groups. e Quantification of the number of WT-1 positive podocytes in glomeruli (left panel), glomerular area (middle panel), and WT-1 positive podocytes/glomerular area (right panel) (n = 7 WT-control; n = 9 WT-NTS; n = 5 Alb-hKL- NTS; n = 9 Pod-hKL-NTS). α-SMA positive area was counted per cortical cross section (10 sections per sample; n = 6 WT-control; n = 10 WT-NTS; n = 5 Alb-hKL-NTS; n = 9 Pod-hKL-NTS). ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. WT-control; @p < 0.05 vs. WT vs. WT-NTS and *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. Alb-hKL-NTS. Scale bars: 50 μm, 30 μm, 5 μm (b), 10 μm, 50 μm (d). Statistical significance between groups was determined using one- way or two-way repeated measures ANOVA followed by multiple comparisons.

    Article Snippet: Sections were washed in PBST (0.1% Triton-X in 1X PBS, pH 7.4) for 10 min before incubation in a sheep polyclonal antibody against nephrin (AF4269, R&D systems) diluted at 1:100 and a rat monoclonal against Klotho (KM2076, Trans Genic Inc.), diluted at 1:200 in 1X PBS with 0.3% Triton X-100 (PBST) at room temperature overnight.

    Techniques: Over Expression, Injection, Control, Staining, Membrane